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Field of application
Separation of peptides according to their physicochemical characteristics.
Coupling to an LTQ-FT ICR mass spectrometer or spotting MALDI target.
Separation principle
The peptides resulting from protein digestion are retained on a column according to its properties (hydrophobicity, ionic force, …).
Applying different solvent gradients allows the progressive elution of these peptides. These gradients are optimized according to the complexity of the sample.
Technical characteristics
Instrument : Ultimate 3000 Dionex
Default configuration :
o C18 Pepmap column, 75 µm i.d., 15 cm, 3 µm, 100A, flow 200 nL/min
o C18 pepmap pre-column, 300 µm i.d., 5 mm, 3 µm, 100A
Automatic sampler 48 tubes or 96 well microplate
Control software : Chromeleon
Installation date : 2009