Assistant Professor ESPCI

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Yann Verdier



Practical course, Molecular Biology, at the ESPCI ParisTech
Tutorial in Biology, at the ESPCI ParisTech


Protein identification by mass spectrometry

keywords :
Immunology ; Alzheimer’s disease ; Reproduction biology
Mass spectrometry (MALDI-TOF, MALDI-TOF/TOF, LC-ESI Orbitrap, LC-ESI FT-ICR)
Biochimie des protéines (immuno-précipitation, électrophorèse 1D et 2D, Western blot)
Molecular biology (DNA and RNA extraction, PCR, RT-PCR, RACE-PCR, cloning, Southern blot, cDNA library, sequencing...)
Bioinformatic : sequence analysis, databank.

Professional Expérience
Since 2006 :
Assistant Professor at the ESPCI ParisTech
Teatching : Biology for 1st year students
Research:Protein identification and characterization, Dr VINH.

2005-2006 :
ATER at the Medical University of Nancy
Teatching : Cell Biology for 1st year student, Pr. FOLIGUET.
Research : Caracterization of transgenic mice, model of Alzheimer’s disease Dr. STRAZIELLE

Post-doc at the Medical University of Szeged (Hungary) ; Pr. PENKE
Identification of membrane proteins involved in interaction between amyloid beta peptide and rat synaptosomes.

PhD These at the Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Nancy ; Dr. BOUE
Selection, identification and caracterisation of fox sperm antigen for their use in a contraceptive vaccine


2012 :
Habilitation à diriger des recherches, University Pierre et Marie Curie, Paris 6.
Proteomic research in Biology : which questions can be asked ?

1998-2002 :
PhD thesis in Biology University Henri Poincaré, Nancy 1.

2001 :
University diploma for Animal experimentation, level 1, University Henri Poincaré, Nancy 1.

2001 :
University diploma Biology applied at the Reproduction, Universities Nancy, Reims, Besançon and Strasbourg.

1998 :
DEA in Medical and Biological Ingenery, University Henri Poincaré, Nancy 1.


[1] Majzoub K, Hafirassou ML, Meignin C, Goto A, Marzi S, Fedorova A, Verdier Y, Vinh J, Hoffmann JA, Martin F, Baumert TF, Schuster C, Imler JL.
RACK1 controls IRES-mediated translation of viruses.
Cell. 2014 Nov 20 ;159(5):1086-95

[2] Fukuyama H*, Verdier Y*, Guan Y, Makino-Okamura C, Shilova V, Liu X, Maksoud E, Matsubayashi J, Haddad I, Spirohn K, Ono K, Hetru C, Rossier J, Ideker T, Boutros M, Vinh J, Hoffmann JA.
Landscape of protein-protein interactions in Drosophila immune deficiency signaling during bacterial challenge.
Proc Natl Acad Sci U S A. 2013 Jun 25 ;110(26):10717-22

[3] Ando K, Dourlen P, Sambo AV, Bretteville A, Belarbi K, Vingtdeux V, Eddarkaoui S, Drobecq H, Ghestem A, Begard S, Demey-Thomas E, Melnyk P, Smet C, Lippens G, Maurage C, Caillet-Boudin ML, Verdier Y, Vinh J, Landrieu I, Galas MC, Blum D, Hamdane M, Sergeant N, Bué L.
Tau pathology modulates Pin1 post-translational modifications and may be relevant as biomarker.
Neurobiology of Aging, 2013 Mar ;34(3):757-69

[4] Fukuyama H, Ndiaye S, Hoffmann J, Rossier J, Liuu S, Vinh J, Verdier Y.
On-beads tryptic proteolysis : an attractive procedure for LC-MS/MS analysis of the Drosophila caspase 8 protein complex during immune response against bacteria
J Proteomics, 2012 ;75(15):4610-9

[5] Brezar V, Culina S, Osterbye T, Guillonneau F, Chiappetta G, Verdier Y, Vinh J, Wong FS, Buus S, Mallone R.
T cells recognizing a Peptide contaminant undetectable by mass spectrometry.
PLoS One. 2011 ;6(12):e28866.

[6] Strazielle C, Jazi R, Verdier Y, Qian S, Lalonde R.
Regional Brain cytochrome oxidase activity in PS1/A246E mutation : correlations with behavioral performances and oxidative stress.
Neurochem. Int. 2009, 55(8):806-814.

[7] Verdier Y, Földi I, Sergeant N, Fülöp L, Penke Z, Janáky T, Szücs M, Penke B.
Characterization of the interaction between Abeta 1-42 and glyceraldehyde phosphodehydrogenase.
J Pept Sci 2008, 14(6) : 755-762.

[8] Verdier Y, Penke B.
Polypeptide and protein aggregates with neurotoxic properties in Neuro-degenerative disorders : emphasis on beta-amyloid –induced dysfunction of receptor-mediated signal transduction pathways.
Current Signal Transduction Therapy 2006, 1(1) : 97-112.

[9] Verdier Y, Huszár E, Penke B, Penke Z, Woffendin G, Scigelova M, Fülöp L, Szucs M, Medzihradszky K, Janáky T.
Identification of synaptic plasma membrane proteins co-precipitated with fibrillar beta-amyloid peptide.
J Neurochem, 2005, 94 (3) : 617-628.

[10] Verdier Y, Farré G, Rouet N, Kele Z, Janáky T, Boué F.
Cloning and sequencing of cDNA encoding for the fox sperm polypeptide Vb of the cytochrome-C oxidase.
J Androl, 2005, 26 (3) : 319-327.

[11] Verdier Y, Farré G, Rouet N, Kele Z, Janáky T, Boué F.
Identification of a testis specific protein, that is a new fox (Vulpes vulpes) sperm antigen localized on the principal piece of the spermatozoa tail.
Biol Reprod, 2005, 72 (2) : 502-508.

[12] Verdier Y, Zarandi M, Penke B.
Amyloid beta-peptide interactions with neuronal and glial cell plasma membrane : binding sites and implications for Alzheimer’s disease.
J Pept Sci, 2004, 10(5) : 229-248

[13] Verdier Y, Penke B.
Binding sites of amyloid ß-peptide in cell plasma membrane and implications for Alzheimer’s disease.
Curr Protein Pept Sci, 2004, 5 (1) : 19-31.

[14] Cliquet F, Verdier Y, Sagné L, Aubert M, Schereffer JL, Selve M, Wasniewski M, Servat A.
Neutralising antibody titration in 25 000 sera of dogs and cats vaccinated against rabies in France , the framework of the new regulations that offer an alternative to quarantine.
Rev Sci Tech Off Int Epiz, 2003, 22 (3) : 857-866.

[15] Verdier Y, Rouet N, Artois M, Boué F.
Partial characterisation of antigenic sperm surface proteins in foxes (Vulpes vulpes).
J Androl, 2002, 23 (4) : 529-536.

[16] Verdier Y, Chaffaux S, Boué F.
Identification of post-vasectomy sperm auto-antigens in fox (Vulpes vulpes) by two-dimensional gel electrophoresis and western blotting.
J Reprod Immunol, 2002, 54 (1-2) : 65-80.

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